A Novel Method of DNA Fingerprint Using Seminal Fluid

DNA fingerprinting is a cornerstone of forensic science, particularly in sexual assault investigations, where biological traces like seminal fluid are critical evidence. Despite advances, challenges remain in obtaining reliable DNA profiles from small or aged semen stains without complex extraction processes. This study proposes a novel and simplified approach using direct PCR amplification of TPOX, TH01, and SRY loci for DNA fingerprinting directly from semen samples and semen-stained materials. A total of 48 semen samples were analyzed through dilution methods and paper-based sample preservation. The results demonstrate that direct PCR without prior DNA extraction provides rapid, cost-effective, and high-quality DNA profiles suitable for forensic applications. The method successfully amplified STR markers even from aged and diluted samples, proving its reliability for criminal investigations. The findings imply that this technique could enhance forensic laboratories' capacity to process sexual assault evidence more efficiently and economically in Iraq and beyond.