Synthesis and Characterization of New Tetrazole Ring Substitutes and Testing of Their Antimicrobial Activity

In this manuscript, several compounds containing a tetrazole ring were produced by reacting hydrazone precursors with sodium azide in a conventional manner using DMF as a solvent. The correctness of the synthesis was confirmed by key organic chemistry assays (FT-IR, ¹H, and ¹³C-NMR), which showed a clear disappearance of azomethine, indicating complete consumption of the reactants. The antimicrobial activity was also evaluated against two widespread bacteria: Staphylococcus aureus and Escherichia coli. The results showed that the inhibition increased with increasing concentration, and preparation (M2) exhibited the highest antimicrobial activity against the bacterial strains used in the study, compared to the antibiotic amepicillin, which served as the control for inhibition. Overall, these compounds have potential for future pharmacological applications.