The Efficiency of Pomegranate Fruit Punica Granatum L Extract in Curing the Resistance of Some Isolated Bacterial Species from Urinary Tract Infection to Antibiotics

Introduction. The growing prevalence of multidrug-resistant bacterial strains poses a major obstacle to the effective management of urinary system infections, as a result of the spread resistance associated plasmids. Objetives. This research investigates the antimicrobial and plasmid-curing potential of watery and alcoholic extracts of P.granatum L. fruits towards six clinically relevant uropathogenic bacteria: Escherichia coli, , Pseudomonas aeruginosa, Staphylococcus aureus ,Proteus vulgaris, Proteus mirabilis, and Klebsiella pneumoniae. Materials and Methods. The antibacterial activity of the tested extracts was rated via the agar well diffusion test, while the minimum inhibitory concentration was determined using the serial dilution method. Sub-minimum inhibitory concentrations of both extracts were used to evaluate their efficacy in curing and removing plasmids. Results. The loss of antibiotic resistance in several isolates was confirmed through phenotypic assays and agarose gel electrophoresis, which revealed the partial or complete disappearance of plasmid DNA bands in treated samples. Conclusions. This research confirm that the pomegranate plant is a promising therapeutic alternative through two mechanisms: its antimicrobial activity against urinary tract pathogens and its role in helping to curb the growing crisis of bacterial resistance to antibiotics. However, this calls for further laboratory and clinical studies on its most active compounds in order to incorporate them into treatment strategies for urinary tract diseases.