Isolation and Identification of Certain Resistant Bacterial Species Causing Bloodstream Infections Associated with Urinary Catheterization and Determination of the Role of Interleukins

This study was conducted on patients suffering from Urinary Catheterization infection in Salah El-Din General Hospital and Tikrit Emergency Hospital from March 2025 to the end of December 2026. The study systematically included 100 participants (30 healthy individuals and 70 patients). Age 20 to 60 years, The study involved the systematic collection of 100 blood and urine samples from all participants. Bacterial isolates, which had been previously cultured on agar media, were subjected to confirmatory identification using the VITEK 2 Compact system. This process strictly adhered to the manufacturer’s instructions provided by the manufacturer. Immunoassays Measurement of Interleukin 6 and Interleukin 8 Concentrations in the enzyme-linked immunosorbent assay (ELISA) technique. Manufacturer: Bioassay Technology; Country of Origin: China. A thorough examination of the quantity and percentage of bacterial isolates found in patients with urinary tract infections, divided out by sex (male and female), is provided by the distribution of bacterial infections by sex in a Urinary Catheterization infection group. The most frequent bacterial isolate, according to the data, is Escherichia coli (14 (35%)). Proteus mirabilis (10 (25%) instances), Klebsiella pneumoniae (7 (17.5%), Pseudomonas aeuroginosa (6 (15%)), and Acinetobacter baumannii (3 (7.5%)). The majority of bacterial infections show gender group disparities (P-value > 0.4) that are not statistically significant. The results demonstrated that serum levels of Interleukin-6 (IL-6) and Interleukin-8 (IL-8) were considerably higher in patients with urinary catheterization infections compared to the healthy control group (P < 0.001). The mean blood level of IL-6 was 49.97 ± 11.67 pg/mL in infected patients and 19.65 ± 9.89 pg/mL in the control group. In a similar vein, the average blood level of IL-8 was 54.12 ± 13.58 pg/mL in the infected people and 22.12 ± 11.65 pg/mL in the controls. These findings demonstrate that pro-inflammatory cytokines are markedly increased in infections linked to urinary catheters, suggesting that the host immune response is triggered during infection.