Evaluates the Active Substances of Extracts of Capsicum Annuum on Enterobius Vermicularis: In Vitro Study
Keywords:
Enterobius vermicularis, Capsicum annuum, secondary compounds, GC-MS, Palmitic acidAbstract
The present investigation was conducted to evaluate the impacts of both cold and hot aqueous extracts of Iraqi Capsicum annuum (C. annuum) fruits on palsy and death of Enterobius vermicularis (E. vermicularis) (in vitro), as well as to identify the main effective secondary compound (alkaloid, terpenoid, and phenolic compounds) of these extracts for analyses by (GC/MS) gas chromatography/mass spectroscopy, along with take-out utmost concentrated compound to assess their impact on the palsy and death of pinworms.
Current findings showed that cold aqueous extract of C. annuum had a high impacts, where its arithmetic rate for palsy and death of pinworms were 474.33 and 619.58 minute, respectively; followed by hot aqueous extract where its drive the palsy and death of pinworms at 577.58 and 773.50 minute , respectively, with a considerable significance difference.
Regarding to the secondary compounds containing in of C. annuum fruits, current study detected that phenolic compounds had greatest impact on E. vermicularis, they cause palsy at 312.50 minute and led to death 417.33 minute. The next compound in order of importance was alkaloid compounds; they are cause palsy at 465.75minute and led to death 621.17 minute. Concerning terpenoid compounds, they are cause palsy at 335.75minute and led to death 446.25 minute, with significance differences.Mebendazole medication had a high effectiveness leading to pinworms' palsy and death an arithmetic rate of 235.00 and 314.00 minute in125 mg/ml concentration, respectively, with a considerable significance differences.
In the context of finding obtaining by GC/MS technique, the current study assured that Iraqi C. annuum fruits contain palmitic acid, with a retention time about 56.010 minutes, where, it had a great efficient with concentration of 125mg/ml, the average arithmetic for palsy about 10 minutes and for death about 15 minute, with significance differences.
