Biochemical and Redox Activity of Curcuma Longa Leaves Naturally Grown in Rodent Model
Downloads
Turmeric, with the Latin name Curcuma longa, is widely known for its extensive medicinal properties, most of which originate from a class of bioactive components inherently contained in the plant itself. A study focused on this plant specifically selected rodents as experimental models, with two items to be detected: one was the various biochemical components contained in the leaves of Curcuma longa, and the other was its antioxidant activity — that is, the redox regulatory capacity mentioned in the original text. The experiment specifically used Wistar strain rats. The researchers divided all the rats participating in the experiment into two equal groups: one was the control group receiving no additional intervention, and the other was the experimental group to observe changes. The daily diet of the experimental group's rats was additionally supplemented with powder ground from Curcuma longa leaves, creating a distinction from the regular diet of the control group. Subsequent biochemical test results showed that these turmeric leaves contained large amounts of phenols, flavonoids and curcuminoids. To further verify its antioxidant capacity, the study also adopted three specialized antioxidant assay experiments: DPPH, ABTS, and FRAP. The final measurement revealed that the turmeric leaves had a strong free radical scavenging ability — and scavenging free radicals is precisely one of the core manifestations of antioxidant effects. Oxidative stress markers, including malondialdehyde, and superoxide dismutase, catalase, and glutathione peroxide assessed in the blood and liver tissue. The experimental group showed low levels of MDA and an increase in SOD, CAT, and GPx activities compared to the control group. The statistical calculations verified the distinguishing difference between the groups with. The outcomes of the research empower the value of antioxidant properties and the ability of Curcuma longa leaves to address issues related to oxidative stress
[1] A. Karthikeyan, C. T. Do Thi, S. C. Anjana, et al., "Turmeric (Curcuma longa L.): the multifaceted golden plant insights and uses," Plant Biotechnology Reports, vol. 19, no. 6, pp. 593–625, 2025, doi: 10.1007/s11816-025-01008-5.
[2] S. Fuloria, J. Mehta, A. Chandel, M. Sekar, N. Rani, M. Begum, V. Subramaniyan, and K. Chidambaram, "A comprehensive review on the therapeutic potential of Curcuma longa Linn. in relation to its major active constituent curcumin," Frontiers in Pharmacology, vol. 13, art. no. 820806, 2022, doi: 10.3389/fphar.2022.820806.
[3] Z. Wang, W. Zhong, W. Zhao, Q. Zhou, et al., "Turmeric: A comprehensive review of its botany, traditional uses, phytochemistry, and mechanisms as a functional food," Nutrients, vol. 18, no. 8, p. 1197, 2026, doi: 10.3390/nu18081197.
[4] J. P. Sahoo et al., "The golden spice turmeric and its feasible benefits in prospering human health—A review," American Journal of Plant Sciences, vol. 12, no. 3, pp. 352–370, 2021, doi: 10.4236/ajps.2021.123030.
[5] K. A. Briseño, J. E. Martínez, E. Y. Hernández-Cruz, and G. E. Chaverri, "Antioxidant effect of curcumin and its impact on mitochondria: Evidence from biological models," Journal of Xenobiotics, vol. 15, no. 5, p. 139, 2025, doi: 10.3390/jox15050139.
[6] M. Cheng, F. Ding, L. Li, C. Dai, X. Sun, J. Xu, F. Chen, M. Li, and X. Li, "Exploring the role of curcumin in mitigating oxidative stress to alleviate lipid metabolism disorders," Frontiers in Pharmacology, vol. 16, art. no. 1517174, 2025, doi: 10.3389/fphar.2025.1517174.
[7] V. L. Singleton, R. Orthofer, and R. M. Lamuela-Raventós, "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent," Methods in Enzymology, vol. 299, pp. 152–178, 1999, doi: 10.1016/S0891-5849(98)00315-3.
[8] C. C. Chang, M. H. Yang, H. M. Wen, and J. C. Chern, "Estimation of total flavonoid content in propolis by two complementary colorimetric methods," Journal of Food and Drug Analysis, vol. 10, no. 3, pp. 178–182, 2002, doi: 10.38212/2224-6614.2748.
[9] G. K. Jayaprakasha, L. J. M. Rao, and K. K. Sakariah, "Improved HPLC method for the determination of curcumin, demethoxycurcumin, and bisdemethoxycurcumin," Journal of Agricultural and Food Chemistry, vol. 50, no. 13, pp. 3668–3672, 2002, doi: 10.1021/jf025506a.
[10] F. Shahidi and A. Samarasinghe, "How to assess antioxidant activity? Advances, limitations, and applications of in vitro, in vivo, and ex vivo approaches," Food Production, Processing and Nutrition, vol. 7, no. 1, p. 50, 2025, doi: 10.1186/s43014-025-00326.
[11] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, "Antioxidant activity applying an improved ABTS radical cation decolorization assay," Free Radical Biology and Medicine, vol. 26, no. 9–10, pp. 1231–1237, 1999, doi: 10.1016/S0891-5849(98)00315-3.
[12] A. Kaushik, C. Jijta, J. J. Kaushik, R. Zeray, A. Ambesajir, and L. Beyene, "FRAP (Ferric reducing ability of plasma) assay and effect of Diplazium esculentum (Retz) Sw. on central nervous system," Indian Journal of Natural Products and Resources, vol. 3, no. 2, pp. 228–231, 2012.
[13] J. Aguilar Diaz De Leon and C. R. Borges, "Evaluation of oxidative stress in biological samples using the thiobarbituric acid reactive substances assay," Journal of Visualized Experiments, no. 159, art. no. e61122, 2020, doi: 10.3791/61122.
[14] C. J. Weydert and J. J. Cullen, "Measurement of superoxide dismutase, catalase, and glutathione peroxidase in cultured cells and tissue," Nature Protocols, vol. 5, no. 1, pp. 51–66, 2010, doi: 10.1038/nprot.2009.197.
[15] M. Vitolo, "Decomposition of hydrogen peroxide by catalase," World Journal of Pharmacy and Pharmaceutical Sciences, vol. 10, no. 8, pp. 47–56, 2021.
[16] H. Mahmoud, A. Abbas, N. Saeed, A. Roaa, and M. Asad, "Enhanced protocol for measuring glutathione peroxidase activity using a new glutathione peroxidase-Tiron assay," Biology Methods and Protocols, vol. 10, no. 1, art. no. bpaf075, 2025, doi: 10.1093/biomethods/bpaf075.
[17] M. Yeni, G. Ganesh, and Y. C. Lee, "Correlation study of antioxidant activity with phenolic and flavonoid compounds in 12 Indonesian indigenous herbs," Antioxidants, vol. 10, no. 10, p. 1530, 2021, doi: 10.3390/antiox10101530.
[18] T. L. N. Gomes, R. S. S. Zenha, A. H. Antunes, F. R. Faria, K. R. Rezende, E. L. de Souza, and J. F. Mota, "Evaluation of the impact of different doses of Curcuma longa L. on antioxidant capacity: A randomized, double-blind, crossover pilot trial," BioMed Research International, vol. 2021, art. no. 3532864, 2021, doi: 10.1155/2021/3532864.
[19] H. Walaa, "Synthesis, characterization, and fluorescence study of new polyesters derived from curcumin analogs," Basrah Journal of Science, vol. 41, no. 3, pp. 521–536, 2023, doi: 10.29072/basjs.20230309.
[20] C. F. Manful, E. Fordjour, D. Subramaniam, A. A. Sey, L. Abbey, and R. Thomas, "Antioxidants and reactive oxygen species: Shaping human health and disease outcomes," International Journal of Molecular Sciences, vol. 26, no. 15, p. 7520, 2025, doi: 10.3390/ijms26157520.
[21] B. Kocaadam and N. Şanlier, "Curcumin, an active component of turmeric (Curcuma longa), and its effects on health," Critical Reviews in Food Science and Nutrition, vol. 57, no. 13, pp. 2889–2895, 2017, doi: 10.1080/10408398.2015.1077195.
Copyright (c) 2026 American Journal of Biomedicine and Pharmacy
This work is licensed under a Creative Commons Attribution 4.0 International License.
