Effect of Pumpkin Seed Oil and Vitamin E on Histological Changes Liver of Rabbite Liver
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The study evaluated the histological and biochemical effects of pumpkin seed oil (PSO), with or without vitamin E, on the liver of male rabbits. Twelve four-month-old rabbits were allocated to three groups (n = 4) and treated for three weeks. Serum ALT and AST activities and hepatic histology were assessed. PSO alone increased ALT (42.53 ± 0.78 U/L) and AST (46.63 ± 4.50 U/L) compared with controls (34.83 ± 0.60 and 33.90 ± 0.21 U/L, respectively), whereas vitamin E supplementation reduced these activities to 27.70 ± 3.15 and 34.70 ± 0.30 U/L. Histologically, PSO induced alterations in liver appearance and central vein diameter, while vitamin E supplementation was associated with reduced biochemical alterations. These findings suggest that vitamin E may mitigate PSO-associated hepatic changes, potentially through antioxidant activity.
[1] T. Fahim, A. Abd-el Fattah, M. Agha, and Z. Gad, “Effect of pumpkin-seed oil on the level of free radical scavengers induced during adjuvant-arthritis in rats,” Pharmacological Research, vol. 31, no. 1, pp. 73–79, 1995, doi: 10.1016/1043-6618(95)80051-4.
[2] M. Gossell-Williams, K. Lyttle, T. Clarke, M. Gardner, and O. Simon, “Supplementation with pumpkin seed oil improves plasma lipid profile and cardiovascular outcomes of female non-ovariectomized and ovariectomized Sprague-Dawley rats,” Phytotherapy Research, vol. 22, no. 7, pp. 873–877, 2008, doi: 10.1002/ptr.2381.
[3] M. Makni, H. Fetoui, N. K. Gargouri, E. M. Garoui, and N. Zeghal, “Antidiabetic effect of flax and pumpkin seed mixture powder: Effect on hyperlipidemia and antioxidant status in alloxan diabetic rats,” Journal of Diabetes and Its Complications, vol. 25, no. 5, pp. 339–345, 2011, doi: 10.1016/j.jdiacomp.2010.09.001.
[4] S. Medjakovic, S. Hobiger, K. Ardjomand-Woelkart, F. Bucar, and A. Jungbauer, “Pumpkin seed extract: Cell growth inhibition of hyperplastic and cancer cells, independent of steroid hormone receptors,” Fitoterapia, vol. 110, pp. 150–156, 2016, doi: 10.1016/j.fitote.2016.03.010.
[5] S. Bardaa, N. Ben Halima, F. Aloui, R. Ben Mansour, H. Jabeur, M. Bouaziz, and Z. Sahnoun, “Oil from pumpkin (Cucurbita pepo L.) seeds: Evaluation of its functional properties on wound healing in rats,” Lipids in Health and Disease, vol. 24, no. 1, Art. no. 148, 2016, doi: 10.1186/s12944-025-02569-8.
[6] M. Yasir, B. Sultana, P. S. Nigam, and R. Owusu-Apenten, “Antioxidant and genoprotective activity of selected Cucurbitaceae seed extracts and LC-ESI-MS/MS identification of phenolic components,” Food Chemistry, vol. 199, pp. 307–313, 2016, doi: 10.1016/j.foodchem.2015.11.138.
[7] B. Rabrenović, B. Dimić, M. Novaković, V. Tešević, and N. Basić, “The most important bioactive components of cold pressed oil from different pumpkin (Cucurbita pepo L.) seeds,” LWT - Food Science and Technology, pp. 521–527, 2014, doi: 10.1016/j.lwt.2013.10.019.
[8] J. S. Seo, B. J. Burri, Z. Quan, and T. R. Neidlinger, “Extraction and chromatography of carotenoids from pumpkin,” Journal of Chromatography A, vol. 1073, no. 1–2, pp. 371–375, 2005, doi: 10.1016/j.chroma.2004.10.044.
[9] L. Nyam, P. Tan, M. Lai, K. Long, and B. C. Man, “Physicochemical properties and bioactive compounds of selected seed oils,” LWT - Food Science and Technology, vol. 42, pp. 1396–1403, 2009, doi: 10.1016/j.lwt.2009.03.006.
[10] A. Nawirska-Olszańska, A. Kita, A. Biesiada, A. Sokół-Łętowska, and A. Z. Kucharska, “Characteristics of antioxidant activity and composition of pumpkin seed oils in 12 cultivars,” Food Chemistry, vol. 139, pp. 155–161, 2013, doi: 10.1016/j.foodchem.2013.02.009.
[11] R. Ahsan, M. Islam, J. I. Bulbul, A. Musaddik, and E. Haque, “Hepatoprotective activity of methanol extract of some medicinal plants against carbon tetrachloride-induced hepatotoxicity in rats,” European Journal of Scientific Research, vol. 37, no. 2, pp. 302–310, 2009.
[12] S. Subramaniam, H. Khan, N. Elumalai, and S. Laksh, “Hepatoprotective effect of ethanolic extract of whole plant of Andrographis paniculata against CCl₄-induced hepatotoxicity in rats,” Comparative Clinical Pathology, pp. 1245–1251, 2015, doi: 10.1007/s00580-015-2067-2.
[13] M. Mapara, S. Thomas, and M. Bhat, “Rabbit as an animal model for experimental research,” Dental Research Journal, vol. 9, no. 1, pp. 111–118, 2012, doi: 10.4103/1735-3327.92960.
[14] W. de Graaf, W. van den Esschert, P. van Lienden, J. Roelofs, and M. van Gulik, “A rabbit model for selective portal vein embolization,” Journal of Surgical Research, vol. 171, no. 2, pp. 486–494, 2011, doi: 10.1016/j.jss.2010.04.032.
[15] S. Seo, H. Oh, H. Lee, T. Ko, and Y. Yoon, “Radiologic anatomy of the rabbit liver on hepatic venography, arteriography, portography, and cholangiography,” Investigative Radiology, vol. 36, no. 3, pp. 186–192, 2001, doi: 10.1097/00004424-200103000-00008.
[16] M. McDowell and F. Trump, “Histologic fixatives suitable for diagnostic light and electron microscopy,” Archives of Pathology & Laboratory Medicine, vol. 100, no. 8, pp. 405–414, 1976.
[17] S. Habib and G. Al-Haaik, “Postnatal renal tubule development in inbred indigenous rabbit (Oryctolagus Cuniculus): A histological and immunohistochemical analysis,” Iraqi Journal of Veterinary Sciences, vol. 39, pp. 79–88, 2025, doi: 10.33899/ijvs.2025.156567.4086.
[18] S. Murugan and B. Kara, “Statistical Packages of Social Sciences,” International Journal of Business and Economics Research, vol. 8, pp. 616–618, 2019.
[19] B. Halliwell and C. Gutteridge, Free Radicals in Biology and Medicine, 5th ed. Oxford, U.K.: Oxford University Press, 2015, doi: 10.1093/acprof:oso/9780198717478.001.0001.
[20] H. Esterbauer, J. Schaur, and H. Zollner, “Chemistry and biochemistry of 4-hydroxynonenal,” Free Radical Biology & Medicine, vol. 11, no. 1, pp. 81–128, 1991, doi: 10.1016/0891-5849(91)90192-6.
[21] M. G. Traber and J. Atkinson, “Vitamin E, antioxidant and nothing more,” Free Radical Biology & Medicine, vol. 43, no. 1, pp. 4–15, 2007, doi: 10.1016/j.freeradbiomed.
[22] H. Sies, “Strategies of antioxidant defense,” European Journal of Biochemistry, vol. 215, no. 2, pp. 213–219, 1993, doi: 10.1111/j.1432-1033.1993.tb18025.x.
[23] B. Fuhrman, N. Volkova, and M. Aviram, “Vitamin E enhances antioxidant activity and protects against oxidative stress,” Nutrition Research, vol. 20, no. 3, pp. 353–359, 2000.
[24] A. Adeel, A. Sohail, and T. Masud, “Characterization and antibacterial study of pumpkin seed oil (Cucurbita pepo),” Life Sciences Leaflets, p. 49, 2014.
[25] G. Stevenson, J. Eller, L. Wang, L. Jane, T. Wang, and E. Inglett, “Oil and tocopherol content and composition of pumpkin seed oil in 12 cultivars,” Journal of Agricultural and Food Chemistry, vol. 55, no. 10, pp. 4005–4013, 2007, doi: 10.1021/jf0706979.
[26] A. Fahmi, N. Hassanen, M. Abdur-Rahman, and E. Shams-Eldin, “Phytochemicals, antioxidant activity and hepatoprotective effect of ginger (Zingiber officinale) on diethylnitrosamine toxicity in rats,” Biomarkers, vol. 24, no. 5, pp. 436–447, 2019, doi: 10.1080/1354750X.2019.1606280.
