Needle Gauge Affecting Recovery and in Vitro Maturation Rates of She-Camel Oocytes
Keywords:
Needle gauge, she-camel, recovery, oocytesAbstract
In this study, she-camel oocytes were obtained from the slaughterhouse in order to determine the impact of needle gauge size on recovery and in vitro maturation rate (IVM). 110 oocytes were collected by aspiration method using two gauges size of needle (18g and 22g). The recovery rate (RR) and the in vitro maturation (IVM) rate were studied. The results showed a superiority of 22g vs the 18g in recovery rate 65/85(76.47%) vs 45/89(51.5%), with a reverse result were noticed in the IVM rate 29/41(70.73%) vs 36/58(62.07%) for 18g and 22g respectively. In conclusion, the use of needle with 18g is the best to use for in vitro maturation (IVM) purposes in she-camel, while when choosing the grade A oocytes only, it is best to use 22g for oocytes recovery.
References
Rose BI. Approaches to oocyte retrieval for advanced reproductive technology cycles planning to utilize in vitro maturation: a review of the many choices to be made. Journal of assisted reproduction and genetics. 2014 Nov;31:1409-19.
Abdulnabi SA, Daham AF. Royal Jelly Improved in Vitro Maturated Bovine Oocytes. Annals of the Romanian Society for Cell Biology. 2021 Apr 20:7653-9.
Hashimoto S, Fukuda A, Murata Y, Kikkawa M, Oku H, Kanaya H, Sonoda M, Sugihara K, Murata T, Nagata F, Nakaoaka Y. Effect of aspiration vacuum on the developmental competence of immature human oocytes retrieved using a 20-gauge needle. Reproductive biomedicine online. 2007 Jan 1;14(4):444-9.
Moawad AR, Ghoneim IM, Darwish GM, Badr MR, El-Badry DA, El-Wishy AB. Factors affecting in vitro embryo production: Insights into dromedary camel. Journal of Animal Reproduction and Biotechnology. 2020;35(2):119-41.
Kushnir VA, Kim A, Gleicher N, Barad DH. A pilot trial of large versus small diameter needles for oocyte retrieval. Reproductive Biology and Endocrinology. 2013 Dec;11:1-5.
Ali A, Benkhalifa M, Miron P. In-vitro maturation of oocytes: biological aspects. Reproductive biomedicine online. 2006 Jan 1;13(3):437-46.
Shirazi A, Ardali MA, Ahmadi E, Nazari H, Mamuee M, Heidari B. The effect of macromolecule source and type of media during in vitro maturation of sheep oocytes on subsequent embryo development. Journal of reproduction & infertility. 2012 Jan;13(1):13.
Singh W, Sonowal J, Das A, Barua P, Gogi C, Mahanta D, Deuri N, Sathapathy S. Recovery of bovine oocytes in respect of quality and quantity by using different techniques. Journal of Entomology and Zoology studies. 2018;6(1):250-3.
Wani AR, Khan MZ, Sofi KA, Malik AA, Lone FA, Bhat FA. EFFECT OF FOLLICULAR SIZE ONIN VITRO MATURATION, FERTILIZATION AND CULTURE OF SHEEP EMBRYOS. Iranian Journal of Veterinary Research, Shiraz University, 2013, Vol. 14, No. 4, Pages 299-304.
Al-dujaily S, Al-Chalabi SM, khadem N, Abdul-mageed M, Abdul lateef MO. Effect of Fertility Blend® Administration on the Oocytes Quality and Embryonic Development using assisted Reproductive Technology in Mice. Journal of Biotechnology Research Center. 2017 Jan 1;11(1):67-71.
Hennet ML, Combelles CM. The antral follicle: a microenvironment for oocyte differentiation. International Journal of Developmental Biology. 2012 Nov 1;56.
Del Campo MR, Del Campo CH, Mapletoft RJ, Ginther OJ. Morphology and location of attached follicular cumulus-oocyte complexes in horses, cattle and llamas. Theriogenology. 1995 Feb 1;43(3):533-42.
Khatir H, Anouassi A. The first dromedary (Camelus dromedarius) offspring obtained from in vitro matured, in vitro fertilized and in vitro cultured abattoir-derived oocytes. Theriogenology. 2006 Jun 1;65(9):1727-36.
Abdelkhalek AE, Gabr SA, Khalil WA, Shamiah SH, Pan L, Qin G, Farouk MH. In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals. Polish Journal of Veterinary Sciences. 2017;20(1).
Wani NA, Nowshari MA. Kinetics of nuclear maturation and effect of holding ovaries at room temperature on in vitro maturation of camel (Camelus dromedarius) oocytes. Theriogenology. 2005 Jul 1;64(1):75-85.
Elsayed MA, Taha NA, Hammam AM, Sawiress FA. Effect of antioxidant supplementation on in vitro maturation of Camelus dromedaries oocytes. Nature and Science. 2015;13(2):17-24.
Petrie A, Watson P. Statistics for veterinary and animal science. John Wiley & Sons; 2013 Feb 28.
Bols PE, Ysebaert MT, Van Soom A, de Kruif A. Effects of needle tip bevel and aspiration procedure on the morphology and developmental capacity of bovine compact cumulus oocyte complexes. Theriogenology. 1997 Apr 15;47(6):1221-36.
Leisinger CA, Coffman EA, da Silva MC, Forshey BS, Pinto CR. Factors affecting in vitro maturation of alpaca (Lama paco) oocytes. Animal reproduction science. 2014 Nov 10;150(1-2):70-5.
Kruip TA, Cran DG, Van Beneden TH, Dieleman SJ. Structural changes in bovine oocytes during final maturation in vivo. Gamete Research. 1983;8(1):29-47.
Davachi ND, Shahneh AZ, Kohram H, Zhandi M, Dashti S, Shamsi H, Moghadam R. In vitro ovine embryo production: the study of seasonal and oocyte recovery method effects. Iranian Red Crescent Medical Journal. 2014 Sep;16(9).
Saleh WM. Assessment of different methods of bovine oocytes collection, maturation and Invitro fertilization of abattoir specimens. Iraqi Journal of Veterinary Sciences. 2017 Jun 28;31(1):55-65.
Nakagawa K, Nishi Y, Kaneyama M, Sugiyama R, Motoyama H, Sugiyama R. The effect of a newly designed needle on the pain and bleeding of patients during oocyte retrieval of a single follicle. Journal of Reproduction & Infertility. 2015 Oct;16(4):207.
